Hi-C analysis of Drosophila melanogaster cells using HiCExplorer

2020, Aug 08    

Introduction

The HiCExplorer is available from the repository suite_hicexplorer. It is a set of tools from deeptools, dedicated towards analysis of data from the 3D conformation of the chromatin.

  1. Map the Hi-C reads to the reference genome
  2. Creation of a Hi-C matrix
  3. Plotting the Hi-C matrix
  4. Correction of Hi-C matrix
  5. TAD Calling
  6. A/B compartments computation
  7. pyGenomeTracks visualization
  8. Loop detection

References

  1. Joachim Wolff, Fidel Ramirez, Vivek Bhardwaj, Ekaterina Polkh, 2020 Hi-C analysis of Drosophila melanogaster cells using HiCExplorer (Galaxy Training Materials). /training-material/topics/epigenetics/tutorials/hicexplorer/tutorial.html Online; accessed Sat Aug 08 2020
  2. Batut et al., 2018 Community-Driven Data Analysis Training for Biology Cell Systems 10.1016/j.cels.2018.05.012